Activation in Bacillus subtilis

Cloning and Enhanced Expression of an Extracellular Alkaline Protease from a Soil Isolate of Bacillus clausii in Bacillus subtilis

in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...

Role of the Sporulation Protein BofA in Regulating Activation of the Bacillus subtilis Developmental Transcription Factor s

Biosynthesis of active Bacillus subtilis urease in the absence of known urease accessory proteins.

Bacillus subtilis contains urease structural genes but lacks the accessory genes typically required for GTP-dependent incorporation of nickel. Nevertheless, B. subtilis was shown to possess a functional urease, and the recombinant enzyme conferred low levels of nickel-dependent activity to Escherichia coli. Additional investigations of the system lead to the suggestion that B. subtilis may use ...

Superdormant spores of bacillus species germinate normally with high pressure, peptidoglycan fragments, and bryostatin.

Superdormant spores of Bacillus cereus and Bacillus subtilis germinated just as well as dormant spores with pressures of 150 or 500 MPa and with or without heat activation. Superdormant B. subtilis spores also germinated as well as dormant spores with peptidoglycan fragments or bryostatin, a Ser/Thr protein kinase activator.

Blue Light Activates the -Dependent Stress Response of Bacillus subtilis via YtvA

Here we present evidence for a physiologically relevant light response mediated by the LOV domaincontaining protein YtvA in the soil bacterium Bacillus subtilis. The loss and overproduction of YtvA abolish and enhance, respectively, the increase in -controlled ctc promoter activity at moderate light intensities. These effects were absent in the dark and in red light but present under blue-light...

Estimation of metabolizable energy equivalency of Bacillus Subtilis spore for male broiler chickens

There are many studies on the effects of probiotics on performance of broiler chickens, but none of them has evaluated the metabolizable energy (ME) equivalency values of probiotics. The aim of this study was to determine the metabolizable energy equivalency value of Bacillus subtilis spore and its potential for decreasing feed ME content and cost. One hundred seventy-six day-old male broilers ...